Journal: SpringerPlus
Article Title: Lysine suppresses myofibrillar protein degradation by regulating the autophagic-lysosomal system through phosphorylation of Akt in C2C12 cells
doi: 10.1186/2193-1801-3-584
Figure Lengend Snippet: Lys suppresses myofibrillar protein degradation through activation of Akt in C2C12 myotubes. Cells were incubated in medium containing DMSO (C), 10 μM Akti (Ai), 10 mM Lys (K), or 10 mM Lys with 10 μM Akti (AiK) for 4 h. The amount of MeHis released from the cells was measured by HPLC. Values are means with SE (n = 3). Different letters indicate significant differences among the groups (p < 0.05).
Article Snippet: Dulbecco’s modified Eagle’s medium (DMEM, low glucose), minimum essential medium (MEM) Vitamin mix, Akti and dimethyl sulfoxide (DMSO) were obtained from Sigma (St. Louis, MO, USA).
Techniques: Activation Assay, Incubation